The Cas9 enzyme must flex and bend in order to bind to the guide RNA (orange). Once the Cas9-RNA complex finds its target DNA (red), the cutting region of Cas9 (yellow) will swing into place relative to its mate (blue) only when the RNA and DNA correctly match. Only then does the enzyme cut the double-stranded DNA.
Samuel Sternberg video
http://www.berkeley.edu
http://www.facebook.com/UCBerkeley
http://twitter.com/UCBerkeley
http://instagram.com/ucberkeleyofficial
https://plus.google.com/+berkeley
Randy W. Schekman, professor of molecular and cell biology at the University of California, Berkeley, has won the 2013 Nobel Prize in Physiology or Medicine for his role in revealing the machinery that regulates the transport and secretion of proteins in our cells. He shares the prize with James E. Rothman of Yale University and Thomas C. Südhof of Stanford University.
http://newscenter.berkeley.edu/2013/10/07/randy-schekman-awarded-2013-nobel-prize-in-physiology-or-medicine/
History 162A, 001 - Fall 2014
Europe and the World: Wars, Empires, Nations 1648-1914 - David Wetzel
Creative Commons 3.0: Attribution-NonCommercial-NoDerivs